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Gene microarray technique and inflammatory arthritis
古洁若
基因微阵列(microarray)检测技术伴随着对人类基因组及其编码的蛋白的相关结构和功能的深入研究,从最初基因的cDNA微阵列,到蛋白微阵列技术等正在迅速发展和广泛应用.我们知道,大多数生物学过程是一个涉及疾病相关基因的识别、量化和蛋白质分子相互作用的复杂网络[1,2],关于基因功能分类的研究也已经存在,如在组织相容性抗原(MHC)和免疫球蛋白系列已较系统和深入.……
基金项目:国家杰出青年科学基金资助项目(30325019)
作者单位:古洁若(510630,广州,中山大学附属第三医院风湿科)
参考文献:
[1]Firneisz G, Zehavi I, Vermes C, et al. Identification and quantification of disease-related gene clusters. Bioinformatics, 2003, 19:1781-1786.
[2]Urbanowaka T, Mangialaio S, Hartmann C, et al. Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease. Cell Biol Toxicol, 2003, 19: 189-202.
[3]Thormton S, Sowdera D, Aronow B, et al. DNA microarray analysis reveals novel gene expression profiles in collagen-induced arthritis. Clin Immunol, 2002, 105: 155-168.
[4]Azuma T, Takei M, Yoshikawa T, et al. Identification of candidate genes for Sjogren′s syndrome MRL/lpr mouse model of Sjogre′ssyndrome and cDNA microanalysis. Immunol Lett, 2002, 81:171-176.
[5]Matthew PV, Constance EB. Early response genes induced in chondrocytes stimulated with the inflammatory cytokine interleukin-1β. Arthritis Res, 2001, 3: 381-388.
[6]Smoot JC, Barbian KD, Van J J, et al. Genome sequence and comparative microarray analysis of serotype M18 group A straptococcus strain associated with acute rheumatic fever outbreaks.Proc Natl Acad Sci USA, 2002, 99: 4668-4673.
[7]Gallagher J, Howlin J, McCarthy C, et al. Identification of Naf1/ABIN-1 among TNF-alpha-induced expressed genes in human synoviocytes using oligonucleotide microarrays. FEBS Lett, 2003,551: 8-12.
[8]Huh SJ, Paik DJ, Chung HS, et al. Regulation of GRB2 and FLICE2 expression by TNF-alpha in rheumatoid synovium. Immunol Lett, 2003, 90: 93-96.
[9]Ruschpler P, Lorenz P, Eichler W, et al. High CXCR3 expression in synovial mast cells associated with CXCL9 and CXCL10 expression in inflammatory synovial tissues of patients with rheumatoid arthritis. Arthritis Res Ther, 2003, 5: R241-252.
[10]Van der Pouw KTC, Van Gaalen FA, Kasperkovitz PT, et al.Rheumatoid arthritis is a heterogeneous disease: evidence for differences in the activation of the STAT-1 pathway between rheumatoid tissues. Arthritis Rheum, 2003, 48: 2132-2145.
[11]Lorenz P, Ruschpler P, Koczan D, et al. From transcriptome to proteome: differentially expressed proteins identified in synovial tissue of patients suffering from rheumatoid arthritis and osteoarthritis by an initial screen with a panel of 791 antibodies.Proteomics, 2003, 3: 991-1002.
[12]Van der Pouw KTC, Van Gaalen FA, Huizinga TW, et al. Discovery of distinctive gene expression profiles in rheumatoid synovium using cDNA microarray technology: evidence for the existence of multiple pathways of tissue destruction and repair. Genes Immun, 2003, 4: 187-196.
[13]Gu J, Rhil M, Marker-Hermann E, et al. Clues to pathogenesis of spondyloarthropathy derived from synovial fluid mononuclear cell gene expression profiles. J Rheumatol, 2002, 29: 2159-2164.
[14]Gu J, Marker-Hermann E, Baeten D, et al. A 588 gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthopathy patients. Rheumatology, 2002, 41: 759-766.
[15]Gu J, Baeten D, Rihl M, et al. Gene Transcripts in the synovia of spondylo-arthropathy (SPA) are clustered into statistical subgroups, each with a unique potential in function, as well as in response to infliximab therapy. Arthritis Rheum, 2002, 45: 435.
[16]古洁若,黄烽,余得恩.cDNA芯片技术在强直性脊柱炎研究中的初步应用.中华医学杂志,2001,81:1030-1034.
[17]古洁若,黄烽,余得恩.髓样细胞核分化抗原(MNDA)在强直性脊柱炎病人的表达.中华微生物和免疫学杂志,2003,23:327-331.
[18]古洁若,黄烽,颜光美,等.Remicade调节强直性脊柱炎患者滑膜细胞基因表达谱的初步研究.中华风湿病学杂志,2003,7:591-595.
收稿日期:2003年12月30日
出版日期:2004年6月15日
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